When I used to work at a local bike shop, the owner of PBI Sprockets gave me a good tip:
get the bike on a centerstand, take the chain guard off, and loosen the axle bolts a little bit so you can hand tighten/loosen the axle nut. Don't loosen the chain adjusters just yet.
you may need a flashlight or something but rotate the wheel and look closely at the rear sprocket/chain. Notice what side the small gap between the sprocket and chain is at.
if you push the chain slop in the opposite direction around the sprocket, rotate the tire, and after a few rotations that gap quickly returns to the original side, you're out of alignment.
Adjust the axle accordingly until the gap between the chain and sprocket doesn't want to immediately go to the left or right side after some rotations.
of course when you're done torque all bolts accordingly.
I've always found this technique helpfull to fine tune the alignment after using the chain block markers as guides. It can be a bit tedious, but after a few times knowing which way and how much to move the axle forward and back forward becomes easier...